@Article{Vainio1978, author = "Vainio, Harri and Zitting, Antti", title = "Interaction of styrene and acetone with drug biotransformation enzymes in rat liver.", journal = "Scandinavian Journal of Work, Environment & Health", year = "1978", month = "VL ", day = "4", number = "2", pages = "47--52", keywords = "acetone; biotransformation enzyme; cytochrome P-450; drug biotransformation; drug biotransformation enzyme; interaction; liver; microsomal enzyme; rat; rat liver; styrene", abstract = "
In the presence of hepatic microsomes, styrene produced a type I difference spectrum, which demonstrates that styrene binds to the catalytic site of ferricytochrome P-450. A comparison of the binding parameters for the interaction of styrene with noninduced, phenobarbital-induced, and 3-methylcholanthrene-induced microsomes indicated that styrene is predominantly bound by cytochrome P-450 and not by cytochrome P-448. Inhalation exposure to a mixture of acetone (1,000 ppm, 6 h/d) and styrene (300 ppm, 6 h/d) for 5 d caused a distinct decrease in hepatic free nonprotein sulfhydryl groups. This decrease could be observed both with and without phenobarbital treatment. Acetone inhalation alone also enhanced ethoxycoumarin O-deethylase activity in rats without pretreatments. Acetone inhalation also increased the cytochrome P-450 content of liver microsomes, but it had no effect on NADPH cytochrome c reductase or epoxide hydratase activity. Combined exposure to styrene and acetone enhanced NADPH cytochrome c reductase activity in nonphenobarbital-treated rats, but no effect was seen in the phenobarbital-treated animals. Phenobarbital treatment of animals can greatly modify the biotransformation and toxicity of styrene, phenobarbital inducible P-450 hemoprotein playing a predominant role in its metabolism. Simultaneous inhalation exposure to acetone also interacts with the metabolism of styrene.
", issn = "0355-3140", doi = "10.5271/sjweh.2736", url = "https://www.sjweh.fi/show_abstract.php?abstract_id=2736", url = "https://doi.org/10.5271/sjweh.2736" }