TY - JOUR T1 - Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing JO - Scandinavian Journal of Work, Environment & Health PY - 1998/6VL - 24 IS - 3 SP - 220 EP - 227 AU - Zhang, X-D AU - Welinder, Hans AU - Jönsson, Bo AG AU - Skerfving, Staffan M3 - doi: 10.5271/sjweh.302 UR - https://www.sjweh.fi/show_abstract.php?abstract_id=302 KW - animal model KW - immunoglobulin E KW - immunoglobulin G KW - occupational allergy KW - structure-activity relationship N2 - '

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OBJECTIVES ': 'The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model.'

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METHODS ': 'The development of specific immunoglobulin (Ig) E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay.'

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RESULTS ': 'The serum titers of specific Ig E after immunization with the different free OAA varied from SN - 0355-3140 ER -